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N Engl J Med 365:327336 de Zeeuw D, Akizawa T, Audhya P, Bakris GL, Chin M, Christ-Schmidt H, Goldsberry A, Houser M, Krauth M, Lambers Heerspink HJ, McMurray JJ, Meyer CJ, Parving HH, Remuzzi G, Toto RD, Vaziri ND, Wanner C, Wittes J, Wrolstad D, Chertow GM (2013) Bardoxolone methyl in type 2 diabetes and stage 4 chronic kidney disease

Measurement of cell viability An MTT assay was conducted to establish the impact of selected flavonoids (C3G, EC, and CH) and positive control (ARB inhibitor) on the murine melanoma cells using CellTiter 96 AQueous One Solution Cell Proliferation Assay Kit (Promega, USA)